Figure 2. Calcium handling is not affected by WNT-5A.

(a) Representative Fura-2 traces (left) and quantification (right) of intracellular calcium (Ca²⁺_i) changes with respect to time of immortalized human airway smooth muscle cells exposed to WNT-5A (500 ng/mL) or histamine (10⁻⁴ M). 30–40 cells were simultaneously measured in the presence of extracellular Ca²⁺ and collectively determined the response. Data represents four independent experiments. *vs pre-stim. (b) Airway smooth muscle cells were pre-incubated with WNT-5A (500 ng/mL) for 48 hours and a dose-response curve to histamine for the maximum Ca²⁺_i peak response was constructed. Data represents four independent experiments. (c) mRNA of airway smooth muscle cells pre-incubated with WNT-5A (500 ng/mL) for 24 hours was isolated and subjected to RT-qPCR. Data represents five independent experiments. Data is expressed as the mean ± SEM.