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. 2016 Jul 29;6:30639. doi: 10.1038/srep30639

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative microphotographs (a,b) and bar graphs (c) show the abundance of TRAP^pos osteoclasts derived from BM-MNCs cultured with TRPV1 agonists (a) capsaicin (10 μM) or olvanil (10 μM) and antagonists (b) capsazepine (10 μM) or SB-366791 (10 μM) under normal (7.4) or low (7.2) pH. Data are expressed as mean ± SEM. *p < 0.05 and **p < 0.01 and ***p < 0.001 vs control at basal pH, ^†p < 0.05 and ^††p < 0.01 vs control acidosis. n = 7 samples/group. Scale bar: 200 μm.