Skip to main content
. 2016 Jul 29;6:30846. doi: 10.1038/srep30846

Figure 1. HCV infection increases c-Myc, HIF-1α, PDK1, and PDK3 levels in human liver.

Figure 1

(a) Representative images of immunohistochemical (IHC) analysis of c-Myc, HIF-1α, PDK1, and PDK3 levels in HCV-non-infected (NHCV) and HCV-infected (HCV) human liver sections. Images of one representative NHCV sample and one HCV liver sample are shown. (b) Comparison of c-Myc, HIF-1α, PDK1, and PDK3 expression in liver sections from NHCV (n = 14) and HCV (n = 14) subjects. IHC signals corresponding to c-Myc, HIF-1α, PDK1, and PDK3 were scored as negative (<5%) or positive (>5%) based on the estimated fraction of stained hepatic tissue showing moderate-to-high signal intensity. (c) Representative immunoblot of c-Myc, HIF-1α, PDK1, PDK3, phospho-PDH Ser293, GK, PHGDH, PSAT-1, and PSPH in NHCV and HCV human liver tissue. β-tubulin was analyzed as an internal control. (d) Relative protein abundances of c-Myc, HIF-1α, PDK1, PDK3, phospho-PDH Ser293, GK, PHGDH, PSAT-1, and PSPH in NHCV and HCV human liver tissue (n = 7 in each group). Immunoblot band intensities are expressed as means ± SEM of three independent experiments. β-tubulin was analyzed as an internal control. *P < 0.001 compared with NHCV. (e) Representative SYBR real-time RT-PCR analysis of expression levels of Myc, HIF-1α, PDK1, PDK3, GK, PHGDH, PSAT-1, and PSPH in normal and HCV-infected human liver tissue. GAPDH mRNA was used as an internal control. Data are means ± SEM of three independent measurements (n = 7 in each group). *P < 0.001 compared with NHCV.