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. 2016 Jul 29;6:30846. doi: 10.1038/srep30846

Figure 4. Pharmacological inhibition of c-Myc and PDK reduces the mRNA and protein levels of enzymes involved in glycolysis and serine synthesis in JFH-1-infected Huh7.5 cells.

Figure 4

(a) Representative immunoblot showing c-Myc, HIF-1α, PDK1, PDK3, GK, PHGDH, PSAT-1, and PSPH levels in JFH-1-infected cells treated with JQ1 (500 nM for 3 days). (b) Representative SYBR real-time RT-PCR analysis of the mRNA expression levels of c-Myc, HIF-1α, PDK1, PDK3, GK, PHGDH, PSAT-1, and PSPH in JFH-1-infected cells treated with or without JQ1 (500 nM, 3 days). GAPDH mRNA was used as an internal control. Data are means ± SEM of three independent measurements from three separate experiments. *P < 0.01, **P < 0.05, ***P < 0.001 compared with uninfected control cells; #P < 0.01, ##P < 0.05, ###P < 0.001 compared with HCV infection alone. (c) Representative immunoblot showing PDK1, PDK3, phospho-PDH Ser293, GK, PHGDH, PSAT-1, and PSPH levels in JFH-1-infected cells treated with DCA (10 mM for 3 days). (d) Representative SYBR real-time RT-PCR analysis of the mRNA expression levels of GK, PHGDH, PSAT-1, and PSPH in JFH-1-infected cells treated with or without DCA. GAPDH mRNA was used as an internal control. Data are the means ± SEM of three independent measurements from three separate experiments. *P < 0.01, **P < 0.05, ***P < 0.001 compared with uninfected control cells; #P < 0.01, ##P < 0.05, ###P < 0.001 compared with HCV infection alone.