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. 2016 Jul 29;6:30632. doi: 10.1038/srep30632

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Copyright © 2016, The Author(s)

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(A) Expression of CSH1 mRNA in HMEC-1 cells exposed to trophoblastic debris (+TD, isolated from 3 individual placentae) for 0, 2, 24 or 48 hours and kept in culture for another 24 hours after withdrawal of a 2 hour exposure. The expression was normalized to the geometric mean of housekeeping genes: RPLP0; ACTB and PPIA. Data are presented as Mean ± SD, P < 0.005. (B) Ct values of CSH1 and housekeeping gene PPIA in trophoblastic debris (isolated from 3 individual placentae) continuously incubated alone for 0, 21 and 48 hours. Note a higher Ct value = low RNA abundance. (C) Newly synthesised RNA extracted from HMEC-1 co-cultured with trophoblastic debris (TD, isolated from 2 individual placentae) for 2 or 24 hours and the matched trophoblastic debris cultured alone for 2 or 24 hours. Expression of CSH1 mRNA was normalized to housekeeping genes (PPIA and ACTB). Data are presented as Mean ± SD.