Table 1. Functional analysis of modulated gene pathways in HMEC-1 treated with trophoblastic debris for 2 and 21 hours.
| Biological process | Count* | %† | P Value | Genes | Fold Enrichment‡ | FDR§ |
|---|---|---|---|---|---|---|
| 2 hours | ||||||
| Cytokine | 15 | 12.6 | 4.00E-12 | CXCL1, CSF2, IL6, CCL3, CCL2, IL-8, CXCL3, CXCL2, CCL4L1, CCL4L2, GREM1, CCL4, LIF, TNFSF10, CCL3L1, CCL3L3, IL1A | 13.74 | 5.02E-09 |
| Anti- apoptosis | 12 | 10.1 | 4.94E-05 | MEF2C, CSF2, IER3, IL6, CCL2, NUAK2, NFKBIA, FOXO1, PIM3, TNFAIP3, BIRC3, IL1A | 4.63 | 0.079 |
| Regulation of transcription | 36 | 30.3 | 9.04E-05 | MEF2C, ACVRL1, NFKBIA, FOXO1, SOX7, CITED4, CNOT4, LIF, REL, BHLHE40, SIK1, IRAK2, ICAM1, NFKBIZ, MAFF, ZNF563, IL6, ERG, EPAS1, CEBPD, ZNF440, RELB, KLF11, NDUFA13, NR4A1, NR4A3, TLE2, C14ORF43, ABCG1, PTHLH, EAF1, NAB2, IRF1, BCL6B, ZSCAN18, KLF4 | 1.89 | 0.145 |
| Leukocyte migration | 6 | 5.0 | 5.59E-05 | 14.38 | 0.090 | |
| 21 hours | ||||||
| Extracellular region | 22 | 28.9 | 7.94E-06 | CSH1, CGA, CSH2, IL-8, INS-IGF2, PSG3, IL32, IGF2, CTSS, GAL, MMP1, VCL, LEP, PSG9, PLIN2, SERPINE1, IL1B, LAMC2, SCG5, TFPI2, DST, ANGPTL4, SPP1 | 2.74 | 0.009 |
| Blood vessel development | 6 | 7.9 | 74.87E-03 | IL-8, ITGAV, IL1B, MKL2, JUNB, ANGPTL4 | 5.34 | 7.396 |
| Response to external stimulus | 6 | 7.9 | 7.37E-04 | LEP, IL-8, OSMR, INS-IGF2, SERPINE1, IGF2, SPP1 | 8.23 | 1.152 |
| Hormone activity | 6 | 7.9 | 1.38E-04 | CSH1, LEP, CGA, CSH2, INS-IGF2, IGF2, GAL | 11.82 | 0.172 |
*Count: Number of genes in the regulated list that were involved in the indicated biological process.
†%: Percentage of the total genes in the annotation category.
‡Fold Enrichment: The ratio of the percentage of involved genes in total regulated genes compared to the background information. The high the score is, the more enrich the biological process is involved in treatment.
§FDR (False Discovery Rate): A statistic for multiple comparison correction.