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. 2016 Jul 29;6:30582. doi: 10.1038/srep30582

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Schematic representation of the Treh locus and the molecular nature of the mutants. Protein-coding regions and untranslated regions are represented by filled boxes and open boxes, respectively. The P-element insertion sites are marked with an inverted triangle. Each transcript is based on information from FlyBase. (B) Sequences of sgRNA target sites and the deletion/insertion of Treh mutants. The 20 bp target sequence corresponding to each target site is indicated in blue and the cleavage sites of Cas9 are shown as triangles. (C) Trehcs1 homozygous mutants were lethal at the pupal period. Trehs1 mutant adult and Trehc1 escaping adult are shown. (D) Lethality of the Treh mutants. Percentages of the indicated mutant adults were determined based on the ratio of heterozygous mutants in each vial. All the values are means and SD (n > 6 [D]).