Fig. 2.

Joint histology of Skap1−/− vs Skap1+/+ (WT) mice. Panel A: Joint histology. Upper panels: Histological features of CIA. Sections of an untreated control (left panel), severely arthritic Skap1+/+ (middle left), mild arthritic CIA-immunized Skap1−/− mouse (middle right) and moderately diseased Skap1−/− mouse (right panel) (×200 magnification). Lower panel: Histogram of percentage of joints. SKAP1-/- (n = 504 joints) and WT (n = 556 joints) mice in each group. Data are from four pooled experiments. ***P < 0.001 compared with the same histological grade in WT mice. (n = 18–22). Panel B: Skap1−/− mice show a reduced numbers of infiltrating T-cells in joints. Immunoperoxidase staining of the tissue sessions with an anti-CD3 Ab. Dark brown cells indicate CD3-positive T cells (×40). Upper panel: images of stained sections; lower panel: histogram showing the number of T-cells in joints on 0–50 scale. At least five areas from each specimen were chosen to determine the numbers of T cell infitrating in each specimen. T cells infiltration of the synovial area of Skap-1-/-mice was significantly reduced, compared with WT mice. P = 0.0005.