Figure 4. Olig2-deletion induces impaired PC differentiation from cerebellar VZ progenitors without affecting proliferation.

(A) Proliferation of cerebellar VZ progenitors at E12.5 is analyzed by their ability to incorporate BrdU in a 2-hour pulse-labeling treatment and compared between Olig2+/+ and Olig2−/− mice. (B) The percentage of BrdU⁺ cells among total cells (DAPI⁺) in the cerebellar VZ (as indicated) is calculated and compared between the two genotypes (n = 3 for both genotypes). (C) PC generation from the proliferating cerebellar VZ progenitors at E12.5 is also analyzed by co-staining of the postmitotic neuronal marker Lhx1/5 and BrdU in Olig2+/+ and Olig2−/− mice. (D) Percentage of Lhx1/5⁺ cells among BrdU⁺ cells in the dorsal portion of the cerebellar VZ is quantified and compared between the two genotypes (n = 3 for both genotypes). VZ, ventricular zone. Scale bar: 20 μm in A and C. *P<0.05 by Student’s t-test.