Figure 5. Differential expression patterns of Olig2 in the VZ progenitors relative to cell cycle in the E12.5 cerebellum and forebrain.

Co-immunostainings were done on mid-sagittal sections of the cerebellum (A) and forebrain (B) of E12.5 embryos that have been pulse-labeled with BrdU for 30 min before sacrifice. (A’,B’) are enlarged images of boxed regions in (A,B), respectively. The arrow indicates a double-positive cell in (B’). (C) Comparison of BrdU labeling in the VZ Olig2⁺ cells between cerebellum and forebrain. Note that BrdU labels more than 50% of the Olig2⁺ cells (BrdU⁺/Olig2⁺) in the GE of the forebrain, but nearly none in the cerebellar VZ. (D) A series of BrdU-pulse labeling analyses of E12.5 cerebellar VZ progenitors. Percentages are shown to represent BrdU⁺ and BrdU^- fractions among Olig2⁺ cells in the cerebellar VZ. VZ, ventricular zone; GE, ganglionic eminence.