Table 3.

Ligand-Induced Change in the BRET Ratio of Wild-Type V2R and Mutants After Stimulation With AVP

	Wild-Type1	NDI Mutants						V2R Polymorphism
		R137H1	Δ From WT2	R181C1	Δ From WT	M311V1	Δ From WT	V266A1	Δ From WT
K-ras	−0.90 ± 0.02a	−0.26 ± 0.01a	0.64 ± 0.05d	−0.05 ± 0.003	0.84 ± 0.02d	−0.68 ± 0.01a	0.22 ± 0.02b	−1.16 ± 0.03a	−0.26 ± 0.03b
Rab1	0.11 ± 0.01a	−0.0002 ± 0.01	−0.11 ± 0.01d	0.003 ± 0.01	−0.11 ± 0.01d	0.11 ± 0.01a	—	0.13 ± 0.05a	0.02 ± 0.05b
Rab4	0.12 ± 0.001a	0.02 ± 0.01	−0.10 ± 0.05d	0.01 ± 0.005	−0.11 ± 0.005d	0.14 ± 0.001a	—	0.17 ± 0.01a	0.05 ± 0.01d
Rab5	0.18 ± 0.001a	0.02 ± 0.01	−0.17 ± 0.01d	0.02 ± 0.005	−0.16 ± 0.01d	0.21 ± 0.01a	—	0.24 ± 0.01a	0.06 ± 0.01c
Rab6	0.01 ± 0.01	0.001 ± 0.003	—	0.004 ± 0.005	—	−0.01 ± 0.03	—	0.02 ± 0.01	—
Rab7	0.07 ± 0.01a	−0.002 ± 0.002	−0.07 ± 0.02d	0.02 ± 0.003	−0.05 ± 0.01d	0.08 ± 0.005a	—	0.10 ± 0.02a	0.03 ± 0.01c
Rab8	0.02 ± 0.01	0.001 ± 0.001	—	−0.005 ± 0.001	—	0.01 ± 0.01	—	0.02 ± 0.01	—
Rab9	0.06 ± 0.001a	0.01 ± 0.004	−0.05 ± 0.005d	0.003 ± 0.01	−0.06 ± 0.001b	0.08 ± 0.01a	—	0.08 ± 0.01a	—
Rab11	0.24 ± 0.03a	0.03 ± 0.01	−0.21 ± 0.03d	0.003 ± 0.004	−0.23 ± 0.03d	0.26 ± 0.02a	—	0.24 ± 0.05a	—

1. Change in BRET ratio from baseline (at t = 0) after AVP (1μM) addition measured at 95 minutes. Values are mean ± SEM of 3 independent experiments. Analysis by two-way repeated measures ANOVA with Sidak's post hoc test for multiple comparisons. 2. Δ from wild-type (WT) indicates a statistically significant increase or decrease in the response after AVP (1μM) addition to or from the indicated compartment marker compared with wild-type V2R response. Analysis by a one-way ANOVA with Dunnett's post hoc test for multiple comparisons. Values are mean ± SEM of 3 independent experiments.

^aP < .001. Change in BRET ratio from own baseline.

^bP < .05. Versus wild type response.

^cP < .01. Versus wild type response.

^dP < .001. Versus wild type response.