Figure 3.

In vitro activation of doxorubicin pro-drug when mixed with HMT. (A) Chemical structures of an alginate monosaccharide modified with tetrazine, doxorubicin, and doxorubicin pro-drug. (B) Sample data from high-pressure liquid chromatography analysis of the supernatant after mixing HTM with doxorubicin pro-drug for 30 min. (C) Cumulative release of doxorubicin after mixing HTM with doxorubicin pro-drug. For HPLC analysis, the concentration of the pro-drug shown at t = 0 min was diluted 10-fold. Data are averages ± SEM, n = 3.