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. 2016 Jul 29;6:30362. doi: 10.1038/srep30362

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Lymphocytes were analyzed with flow cytometry after stimulation with PMA/ionomycin and BFA for 5 h. (A) FACS gating was used in the CD8⁺ T cell analysis. (B,C) Representative FACS data and summary analysis show the IFN-γ, IL-17 and IL-4 co-expression with IL-21 by CD8⁺ T cells from polyp (n = 30) and control uncinate tissues (n = 19). (D) Representative FACS data and summary results show the CD45RO, CD62L and CCR7 expression in IL-21-expressing CD8⁺ T cells from polyp tissues (n = 30). The statistical significance was determined with the Mann–Whitney U test. ***P < 0.001. NP, nasal polyp; UN, uncinate.