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. 2016 Feb 9;19(7):pyw011. doi: 10.1093/ijnp/pyw011

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© The Author 2016. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Effects of chronic morphine administration and withdrawal on body weight and relative spleen weight. Male C57BL/6J mice were treated with either saline or escalating-dose morphine paradigm (2 x 20–100mg/kg/d, i.p.) followed by a 7-day withdrawal period. (A) Changes in body weight and (B) relative spleen weight during chronic morphine or saline administration and withdrawal. Data are expressed as the mean ± SEM (n=6–12/group). *P<.05, **P<.01, ***P<.001 vs saline control (repeated-measures 2-way ANOVA followed by Bonferroni post-hoc test).