Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jun 12;3(4):223–229. doi: 10.1093/rb/rbw023

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — C₁₂-PEI/SPIO nanocomposites induced autophagy can be prevented by PEI lactosylation. (a) Western blotting assay of RAW 264.7 cells that were untreated (negative control), treated with rapamycin (50 μg/ml, 8 h), LPS (1 μg/ml 24 h) and nanocomposites (0.5, 2.5, 5 μg/ml, 24 h). Both rapamycin and LPS groups served as positive controls. The RAW 264.7 cells stably expressing GFP-LC3 were labelled with nanocomposites at an iron concentration of 5 μg/ml for 24 h. (b) the GFP puncta in RAW 264.7 cells were measured and summarized. Data were the mean value of three independent experiments with each count of no less than 60 cells. Values are expressed as the mean ± SD, n = 3. (c) the representative images of GFP puncta in RAW 264.7 cells after treatment under a CLSM. Scale bar: 10 μm.