(A) mRNA levels of Il21,
Il21r, Stat3, caspase 3, and Bcl2 from Il21r WT and Il21r KO were determined by qRT-PCR 12 hours and 24 hours after 30 minutes of OD. Gapdh was used for normalization. Experiments were performed 3 times using 3 mice per genotype for each experiment. Values represent mean ± SEM. (B) Western blots were performed to detect both cleaved caspase 3 (c–caspase 3) and BCL-2 in explanted brain slices of Il21r WT (+/+) and Il21r KO (–/–) 12 and 24 hours after 30 minutes of OD. (C) Levels of both cleaved caspase-3 and BCL-2 protein were determined. α-Tubulin was used for normalization. Experiments were performed 4 times using 3 mice per genotype for each experiment. Values represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, 2-tailed Student’s t test.