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. 2016 Jul 11;126(8):2827–2838. doi: 10.1172/JCI84491

Figure 4. Il21r KO increases cleaved caspase 3 after ischemic stroke stimulation in an ex vivo brain slice stroke model.

Figure 4

(A) mRNA levels of Il21, Il21r, Stat3, caspase 3, and Bcl2 from Il21r WT and Il21r KO were determined by qRT-PCR 12 hours and 24 hours after 30 minutes of OD. Gapdh was used for normalization. Experiments were performed 3 times using 3 mice per genotype for each experiment. Values represent mean ± SEM. (B) Western blots were performed to detect both cleaved caspase 3 (c–caspase 3) and BCL-2 in explanted brain slices of Il21r WT (+/+) and Il21r KO (–/–) 12 and 24 hours after 30 minutes of OD. (C) Levels of both cleaved caspase-3 and BCL-2 protein were determined. α-Tubulin was used for normalization. Experiments were performed 4 times using 3 mice per genotype for each experiment. Values represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, 2-tailed Student’s t test.