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. 2016 Jul 18;126(8):3089–3103. doi: 10.1172/JCI83404

Figure 1. p21 controls in vivo endotoxin tolerance.

Figure 1

(A) P21–/– and WT mice (n = 6) were treated with a single, high LPS dose, and showed 100% death (left). To induce tolerance, P21–/– and WT mice (n = 10) received a low LPS dose, followed by a high dose after 16 hours. P21–/– mice did not develop tolerance compared to WT mice, as indicated by a Kaplan-Meier survival curve (right). **P < 0.01, log‑rank (Mantel-Cox) test. (B) Blood was collected 2 hours after the second LPS injection and serum cytokines measured by ELISA. Levels of M1-associated cytokines (TNF-α and IFN-β) were elevated in the serum of P21–/– compared with WT mice. Data show the mean ± SD (n = 3 mice), *P < 0.05, 2-tailed Student’s t test.