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. 2016 Jun 27;126(8):2970–2988. doi: 10.1172/JCI85456

Figure 2. c-Abl overexpression decreases survival of hA53Tα-syn transgenic mice.

Figure 2

(A) Breeding strategy to generate BCR-ABL hA53Tα-syn PrP-tTA trigenic mice. (B) Kaplan-Meier survival curve analysis for hA53Tα-syn, PrP-tTA hA53Tα-syn, BCR-ABL hA53Tα-syn PrP-tTA trigenic, and BCR-ABL hA53Tα-syn PrP-tTA trigenic mice with doxycycline (n = 20–30 mice per group). Statistical analysis was performed by Mann-Whitney-Wilcoxon test. (C, E, and G) Representative immunoblots of BCR-ABL, pY245 c-Abl, α-syn, and β-actin in the brain stem, spinal cord, and cortex from 3-month-old BCR-ABL hA53Tα-syn PrP-tTA trigenic mice with or without doxycycline and age-matched littermate controls. (D, F, and H) Quantification of BCR-ABL, pY245 c-Abl, and α-syn protein levels normalized to β-actin (n = 3 per group). Data are from 3 independent experiments. Statistical significance was determined by 1-way ANOVA with Tukey’s post-test of multiple comparisons. Quantified data are expressed as the mean ± SEM. **P < 0.01, ***P < 0.001.