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. 2016 Jun 27;126(8):2970–2988. doi: 10.1172/JCI85456

Figure 8. Phosphotyrosine 39 α-synuclein level is elevated in PD patients and accumulates in Lewy bodies.

Figure 8

(A, C, and E) Representative immunoblots of c-Abl, pY245 c-Abl, pY39 α-syn, pS129 α-syn, α-syn, and β-actin in the total lysates from substantia nigra, striatum, and cortex regions from PD patient brains and age-matched controls. (B) Quantification of c-Abl protein level normalized to β-actin, pY245 c-Abl protein level normalized to c-Abl, and pY39 α-syn and pS129 α-syn protein levels normalized to α-syn in the substantia nigra (control n = 5, PD n = 8). (D and F) Quantification of pY245 c-Abl protein level normalized to c-Abl and pY39 α-syn and pS129 α-syn protein levels normalized to α-syn in the striatum (control n = 5, PD n = 7) (D) and cortex (control n = 7, PD n = 6). Data are from 3 independent experiments. Statistical significance was determined by 2-tailed unpaired Student’s t test. Quantified data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01. (G and I) Representative pY39 α-syn and pS129 α-syn IHC in the substantia nigra from PD patient brains and age-matched controls (n = 2). Scale bars: 250 μm. (H and J) High-magnification images of G and I. Scale bars: 25 μm.