Figure 8. p53-sestrin interactions coordinate AMPK and mTORC1 signaling in human decidual cells.

(A–D) Met or Rsv inversely regulated pAMPK and pS6 levels in HuF cells (n = 3, *P < 0.05, mean ± SEM, Student’s t test). (E–J) pAMPK and pS6 levels were inversely regulated by Met or Rsv in human primary decidual cells isolated from term placentas. Each sample was isolated from a discrete decidua from a single patient. pAMPK and pS6 levels were inversely regulated by Met or Rsv (n = 4, *P < 0.05, mean ± SEM, ANOVA). (K and L) SESN1 and SESN2 mRNA was upregulated by P₄ in term decidual cells, but not in preterm decidual cells (n = 3, *P < 0.05, mean ± SEM, Student’s t test).