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. 2016 Jul 18;126(8):3036–3052. doi: 10.1172/JCI83416

Figure 5. Assessment of sustenance of the antitumor activity of TanCAR T cells and their expression of the exhaustion markers PD-1, LAG3, and TIM3.

Figure 5

(A) Continuous graphical output of cell index values up to the 150-hour time point from U373 during incubation with TanCAR, biCAR, CARpool, HER2 CAR, and IL13Rα2 CAR T cells and tumor only using the xCELLigence impedance system. U373 cells were seeded in electrode-coated 96-well plates (e-plates) in triplicates. T cells were added at a ratio of 1 T cell for each 10 U373 tumor cells 24 hours later to allow for U373 attachment and confluence. Electrical impedance was recorded continuously as an indicator of U373 density. (B) Median fluorescence intensity values for PD-1, LAG3, and TIM3 expression on the CD8+ and CD4+ CAR T cell compartment before and after repeated stimulation with U373 tumor cells for 1 week. For both experiments, shown are representative data from 3 independent experiments done in triplicates. A single-step Tukey’s range test was used. *P < 0.05, **P < 0.005.