Table 1.
Experimental method settings with systematic variation of several parameters. “+” sign indicates high source voltage; long MS1/MS2 scan time/accumulation; or high MS2 precursor selection threshold. “−” sign corresponds to lower settings. M0 is a MS1 only method, thus the MS2 parameters are listed as “NA” (not applicable)
| Method ID | Source Voltage | MS1 Scan Time | MS2 Scan Time | Selection Threshold |
|---|---|---|---|---|
| M0 | − | + | NA | NA |
| M1 | − | − | + | − |
| M2 | + | − | + | − |
| M3 | − | + | + | − |
| M4 | − | − | − | − |
| M5 | − | − | + | + |
| M6 | − | + | − | − |
| M7 | − | + | + | + |
| M8 | − | − | − | + |
| M9 | − | + | − | + |