TABLE 1.
Primers used in this study
| Function and primer | Sequencea | PCR product size (bp) |
|---|---|---|
| Construction of arsR FA1090 opaA-K strain | ||
| arsR-Kan-Fw | TACCGCGATACCCCTGTAAC | 2,211 |
| arsR-Kan-Rv | ACACGTTGGCACAACTGGAC | |
| Construction of arsR-complemented strain | ||
| arsR-PacI-Fw | GGGGGGTTAATTAAGTAGGGGCGGCAGTTTTATG | 698 |
| arsR-FseI-Rv | TTTTTTGGCCGGCCGGAAATTTGTCCTGAT | |
| Construction of His-tagged protein | ||
| NdeI_arsR_Fw | CATATGAACACAATACCGCTCCAC | 291 |
| BamHI_arsR_Rv | GGATCCATTCTAACACTATGCCCG | |
| RT-PCR | ||
| arsR-RT-Fw | ATGAACACAATACCGCTCCA | 191 |
| arsR-RT-Rv | TAACGCGTAAAGTCCACCAG | |
| fbpA-RT-Fw | CTCCACCATCAACGAAACAC | 216 |
| fbpA-RT-Rv | GCGACAATCTGTTCCAAGAA | |
| Cloning of promoter regions | ||
| BamHI-arsR-p-Fw | CCCCCCGGATCCAGGGTAGGGGCGGCA | 271 |
| BamHI-arsR-p-Rv | CCCCCCGGATCCGGATCAGTATCGCCA | |
| BamHI-fur-p-Fw | CCCCCCGGATCCGGGTTTTGTTC | 256 |
| BamHI-fur-p-Rv | CCCCCCGGATCCCAATACCCCTGTA | |
| Competition assay | ||
| fur forward | CTGCTTGAATATTTTATAAAAGCGAACGATAATCATACGCTTAAGCGGT | 49 |
| fur complemented | ACCGCTTAAGCGTATGATTATCGTTCGCTTTTATAAAATATTCAAGCAG | |
| rmp forward | TAGTGGAGACTGAAATATCCGATTTGCCGCCATGTTTCTACAGCGGCCTG | 50 |
| rmp complemented | CAGGCCGCTGTAGAAACATGGCGGCAAATCGGATATTTCAGTCTCCACTA | |
| ArsR binding assay | ||
| norB-p forward | GAATAATGATAGTTATTATCATATATGTATTTGTTCTATATGTGAGTTTG | 50 |
| norB-p complementary | CAAACTCACATATAGAACAAATACATATATGATAATAACTATCATTATTC | |
| GC-rich forward | GCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGC | 50 |
| GC-rich complementary | GCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGCTGGC |
a
Underlining indicates restriction enzyme sites.