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. 2016 Jul 28;198(16):2192–2203. doi: 10.1128/JB.00329-16

FIG 2.

FIG 2

Complementation test of LptC depletion mutants with different E. coli and P. aeruginosa wild-type or chimeric LptC constructs. Cultures of FL905 (araBp-lptC) strains freshly transformed with pGS100 derivatives expressing Ec-LptC (pGS103, CCC), Pa-LptC (pGS111, PPP), LptC chimeras (pGS201, CPP; pGS202, PCC; pGS203, CPC; pGS204, PCP; pGS206, CCP; pGS207, PPC), or a truncated Ec-LptC protein missing region 3 (pGS208, CCΔ) and grown in LD-chloramphenicol-arabinose were serially diluted 1:10 in microtiter wells and replica plated in agar plates with (+ ara) or without (− ara) arabinose or with glucose (+ glu) to fully repress the araBp promoter. The log of the serial dilutions is indicated on the right of the panel.