FIG 6.
Rescue of FL905 growth by overexpression of truncated Ec-lptC or Pa-lptC alleles. FL905 cells transformed with pGS100 (−) or pGS100 derivatives harboring wild-type LptC (pGS103), LptCΔ139-191 (pGS417), LptC190N (pGS408), or Pa-LptC (pGS111), as indicated on top of the lanes, were grown in LD-chloramphenicol-arabinose, serially diluted 1:10 in microtiter wells, and replica plated on LD-agar plates with chloramphenicol and with (+ ara) or without (− ara) arabinose and with IPTG (+ IPTG) to induce the lptC allele on the plasmids, as described in Materials and Methods. The log of the serial dilutions is indicated on the right.