TABLE 3.
Complementation assays in LptC-depleted cells with additional ectopic expressiona of lptA-lptB
| Suppressing gene | Efficiency of plating with complementing lptC allele: |
|||||
|---|---|---|---|---|---|---|
| None | Ec-lptC | Ec-lptC190N | Ec-lptCΔ139-191 | Ec-lptCG153R | Pa-lptC | |
| None | − | + | − | − | − | − |
| lptAB | − | + | + | + | − | ± |
| lptA | − | + | − | − | − | − |
| lptB | − | + | + | + | − | + |
a
FL905 strains harboring pGS401 derivatives expressing different combinations of lptC complementing alleles and lptA-lptB suppressor genes were grown in LD with 0.2% arabinose and 25 μg/ml chloramphenicol. Serial dilutions in microtiter plates were replica plated on the same medium or without arabinose. Efficiency of plating in the nonpermissive condition (no arabinose) is expressed as follows: +, about 1; ±, between 10−2 and 10−3; −, <10−3.