TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAK | Strain PAK | 15 |
| PAK pilA | PAK with a deletion of pilA | 60 |
| PAK pilU | PAK with a deletion of pilU | Matthew C. Wolgang |
| PAK cyaAB | PAK with deletions of cyaA and cyaB | 15 |
| PAK cyaB-R456L | PAK encoding an arginine-to-leucine change in CyaB at amino acid position 456 | This study |
| PAK fimV | PAK with a deletion of fimV | 15 |
| PAK fimV cyaB-R456L | PAK carrying a deletion of fimV and encoding an arginine-to-leucine change in CyaB at amino acid position 456 | This study |
| PAK fimV1194 | PAK with an FRT insertion at nucleotide position 1194 of fimV | This study |
| E. coli | ||
| BL21(DE3) | Protein overexpression strain | Invitrogen |
| B834(DE3) | Methionine auxotrophic protein expression strain | Invitrogen |
| BTH101 | Bacterial two-hybrid system reporter strain | Euromedex |
| Plasmids | ||
| pRK793 | IPTG-inducible expression vector encoding TEV protease | 41 |
| pBADGr | Arabinose-inducible protein expression vector | 61 |
| pBADGr::fimV | Arabinose-inducible vector encoding full-length FimV | This study |
| pBADGr::fimV689 | Arabinose-inducible vector encoding FimV residues 1 to 689 | This study |
| pET151/D-TOPO::fimV862–919 | IPTG-inducible protein overexpression vector encoding FimV residues 862 to 919 | This study |
| pKNT25 | IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB | Euromedex |
| pKNT25::fimV488–618 | IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR1+2 | This study |
| pKNT25::fimV811–919 | IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR3 | This study |
| pKNT25::fimL | IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of FimL | This study |
| pKNT25::pilG | IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of PilG | This study |
| pUT18 | IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB | Euromedex |
| pUT18::fimV488–618 | IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR1+2 | This study |
| pUT18::fimV811–919 | IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR3 | This study |
| pUT18::fimL | IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB fused to the C terminus of FimL | This study |