TABLE 2.

TCA cycle enzyme essentiality

TCA cycle enzyme	Essentialitya in:
	R. gelatinosus	R. palustrisb	C. crescentusc	B. subvibrioidesd	A. tumefaciensd	P. aeruginosae
Citrate synthase	U	E	N	N	E	E
Aconitase	E	E	E	E	N	N
Isocitrate dehydrogenase	N	E	E	N	E	N
α-Ketoglutarate dehydrogenase	E	E	E	E	E	E
Succinyl-CoA synthetase	N	N	E	E	E	N
Succinate dehydrogenase	U	E	E	E	E	E
Fumarase	E	N	E	E	E	E
Malate dehydrogenase	N	E	E	E	N	N
Total no. of essential enzymes	3	6	7	6	6	4

^aEssentiality is reported as essential (E), nonessential (N), or unresolved/high fitness (U).

^bDesignations are based on reference 21.

^cDesignations are based on reference 14.

^dDesignations are based on reference 15.

^eDesignations are based on reference 16.