FIG 4.

Flagellar rotation and motility promote OMV production. (A) Fold difference, relative to the wild type (WT), in the level of OMV production by four motility mutants: the flrA mutant (no flagellum), the motB1 mutant (nonrotating flagellum), the cheA mutant (chemotaxis defect), and a hyperflagellated swarmer mutant (HS). (B) Influence of medium viscosity (i.e., addition of 0, 10, or 20% Percoll) on relative levels of OMV production. Values are means ± standard errors of the means (SEM; n = 3). ****, P < 0.0001; ***, P < 0.001; **, P < 0.01; *, P < 0.1; ns, not significant.