FIG 2.

(A to D) Growth and substrate consumption in minimal medium with 0.3% (wt/vol) glucose plus 0.2% (wt/vol) Neu5Ac (A and B) or with 0.3% (wt/vol) fructose plus 0.2% (wt/vol) Neu5Ac (C and D) of WT C. glutamicum (A and C) and C. glutamicum ΔnanR (B and D). Solid circles, growth; open triangles, Neu5Ac; solid squares, glucose; open diamonds, fructose. (E) Growth of WT C. glutamicum (solid circles) and C. glutamicum ΔnanR (open triangles) in minimal medium with 0.2% (wt/vol) Neu5Ac. Three independent cultivations were performed; data from one representative experiment are shown. Results of all of the cultivations were comparable. (F) Rates of [¹⁴C]Neu5Ac uptake of WT and ΔnanR C. glutamicum cells cultivated in minimal medium with different substrates. Data represent mean values and standard deviations of three independent measurements from two independent cultivations.