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. 2016 Jul 28;198(16):2204–2218. doi: 10.1128/JB.00820-15

FIG 3.

FIG 3

(A) Analyses of siaE promoter activity, measured as relative fluorescence of the GFP reporter upon expression of the gfp gene under the control of the siaE promoter in C. glutamicum(pEPRI-PRsiaE) and C. glutamicum ΔnanR(pEPRI-PRsiaE) cells cultivated for 8 h in minimal medium plus glucose, Neu5Ac, or fructose. Data represent mean values and standard deviations of two independent measurements each from three independent cultivations. (B) Growth of C. glutamicum ΔnanR (open diamonds), C. glutamicum(pEKEx2) (solid circles), and C. glutamicum (pEKEx2-siaEFGI) (gray triangles) in minimal medium with 0.2% (wt/vol) Neu5Ac. (C) Rates of [14C]Neu5Ac uptake of C. glutamicum(pEKEx2) and C. glutamicum(pEKEx2-siaEFGI) cells cultivated in minimal medium with 0.3% (wt/vol) fructose. Data represent mean values and standard deviations of three independent measurements from two independent cultivations.