FIG 6.

Electrophoretic mobility shift assays with NanR and probes covering the nanR-siaE intergenic region. DNA probes containing the nanR-siaE intergenic region were incubated with various concentrations of NanR. (A) Schematic illustration of the intergenic region of the C. glutamicum nanA and siaE genes, probes used for EMSAs, and localization of the NanR-binding site. (B) Representative EMSAs using 0.2 μg of NanR with 10 ng of each of the probes PnanR, PnanR-A, PnanR-B, and PnanR-C. (C) Representative EMSA using 0.2 μg NanR with 10 ng of the probe PnanH, which covers the nanH promoter. As a negative control, 10 ng of a PCR product of the nanR gene generated with the primers OE_nanR_fwd and OE_nanR_rev was used.