FIG 7.

(A and B) Growth (black circles), substrate concentrations (white triangles, Neu5Ac; black squares, fructose), and relative fluorescence of the GFP reporter (gray bars) in cultivations of C. glutamicum WT (pEPRI-PRsiaE) on minimal medium with 0.2% (wt/vol) Neu5Ac (A) or 0.2% (wt/vol) Neu5Ac plus 0.25% (wt/vol) fructose (B) as substrates. (C) Analyses of siaE, nanA, nagA, and nanR promoter activities in WT C. glutamicum and C. glutamicum ΔnanR strains after 5 h of cultivation in minimal medium with 0.2% (wt/vol) Neu5Ac plus 0.4% (wt/vol) fructose (white boxes) or in minimal medium with 0.2% (wt/vol) Neu5Ac (gray boxes). Three independent cultivations were performed. Growth data from one representative experiment are shown; results of all of the cultivations were comparable. GFP fluorescence data represent mean values and standard deviations of two independent measurements each from three independent cultivations.