TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | F− ϕ80lacZΔM15 Δ(lacZYA-argF)U169 phoA supE44 hsdR1 recA1 endA1 gyrA96 thi-1 relA1 | 89 |
| BL21(DE3) | ompT hsdSB(rB− mB−) gal dcm (DE3) | 90 |
| C. glutamicum strains | ||
| ATCC 13032 | Wild type | American Type Culture Collection |
| ΔnanR mutant | WT C. glutamicum with deletion of nanR (cg2936) | This work |
| Plasmids | ||
| pEKEx2 | Expression vector; ptac lacIq Kmr | 91 |
| pEKEx2-nanR | pEKEx2 carrying the nanR (cg2936) gene | This work |
| pEKEx2-siaEFGI | pEKEx2 carrying the genes siaE (cg2937), siaF (cg2938), siaG (cg2939), and siaI (cg2940) | This work |
| pK19mobsacB | Kmr; mobilizable E. coli vector for the construction of insertion and deletion mutations in C. glutamicum (oriV sacB lacZα) | 51 |
| pK19mobsacBΔnanR | Kmr; pK19mobsacB with the deletion construct for the gene nanR (cg2936) | This work |
| pASK_IBA3 | Expression vector, tetA promoter, C-terminal Strep-tag II; Ampr | IBA GmbH |
| pASK_IBA3_nanR | pASK_IBA3 carrying the nanR gene | This work |
| pEPRI | Promoter probe vector carrying the promoterless gfp gene; Kmr | 52 |
| pEPRI_PRnagAB_WT | pEPRI containing the nagA (cg2929) promoter fragment from C. glutamicum ATCC 13032 | 24 |
| pEPRI_PRnagAB_M4 | pEPRI containing the nagA (cg2929) promoter fragment from C. glutamicum M4 | 24 |
| pEPRI-PRnanA | pEPRI containing the nanA (cg2931) promoter fragment | This work |
| pEPRI-PRsiaE | pEPRI containing the siaE (cg2937) promoter fragment | This work |
| pEPRI-PRnanH | pEPRI containing the nanH (cg1756) promoter fragment | This work |
| pEPRI-PRnanR | pEPRI containing the nanR (cg2936) promoter fragment | This work |