TABLE 1.

N. gonorrhoeae strains used in this study

Strain name or genotype	Description	Source or reference
FA1090NV	FA1090 1-81-S2 with a nonvariant pilE G4 mutation (pilENV) and a pilC1 phase-locked allele	6
pilT::erm	FA1090 harboring pilENV and pilT::erm alleles	6
Opaless	pilENV strain containing loss-of-function mutations in all 11 opa alleles	39
Opaless, OpaD+	Opaless strain harboring a nonvariable copy of the opaD gene	39
Opaless, ΔpilE	Opaless strain harboring pilE deletion allele	39
Opaless, pilT::erm	Opaless strain harboring the pilT insertion allele	This study
Opaless, OpaD+ pilT::erm	Opaless, OpaD+ strain harboring the pilT insertion allele	This study