FIG 3.

HPLC analysis of cobamide extracts from S. enterica JE7088 mutant strains. Strain JE11685/pBAD24 and strain JE20971 ΔcobD/pBAD24+SMUL_1544 were analyzed. l-Ser-P or l-Thr-P was added to the culture of JE20971 at a concentration of 1 mM. Pseudo-B₁₂ extracted from Propionibacterium acidipropionici and norpseudo-B₁₂ extracted from S. multivorans served as standards. The suffix vitamin refers to the presence of a cyano group as the upper ligand in the extracted cobamides. mAU, milli-absorbance units.