Figure 8.
Therapeutic evaluation of mAb 3E7b against CHIKV-induced lethality in a neonate mouse model. Six-day old BALB/c mice were i.p injected with CHIKV of 4 × 105 PFU prior to administration of PBS, 20 μg of IgM isotype control or 3E7b at 8 h or 4 h p.i. (A) Survival (%) till 14 day p.i. is presented as Kaplan-Meier survival curves and statistically analyzed by log-rank test (GraphPad Prism 6). (B–F) Neonate mice were CHIKV-infected as described and post-treated with 20 μg of 3E7b at 4 h p.i. At day 2 p.i., serum and various tissues were harvested and quantitated for CHIKV titer. Thick line represents the mean virus titer and dashed line indicates the limit of the detection. Kruskal-Wallis test was performed across all treatment followed by a Dunn's post test to PBS infected control. Statistical significance is defined as *p < 0.05 and **p < 0.01 (GraphPad Prism 6).