Figure 2.

Flow cytometric analysis of PBMC from DD9 After magnetic B cell enrichment, a gate was placed to eliminate debris, followed by a gate on the viable CD19⁺, then CD27⁺ followed by surface IgG⁺. Gated cells were then analyzed for binding to DEN-2–80E (C). Separate stains of the same sample were performed as controls. The cells were analyzed for binding to DEN-2–80E following pre-incubation with 100X concentrated unlabeled DEN-2–80E (D) or analyzed for binding to the secondary SA-PE without DEN-2–80E (B).