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Quantification of DEN-2–80E specific B memory cells in the direct flow cytometry and cultured B ELISPOT assays PBMC from dengue seropositive donors (open circles) and controls (filled circles) were tested in the direct flow cytometry assay (A) and the 6-day cultured B ELISPOT (B) for the presence of DEN-2 80E memory B cells. The lines on each graph represent the group mean with SEM.
