Table 3.
Residues critical for mAb binding
| Mutation | DB5 Fab | DB5 MAb | DC4 Fab | DC4 mAb | DD1L Fab | DD1L MAb | DC11 Fab | DC11 mAb | Control Mab D11C |
|---|---|---|---|---|---|---|---|---|---|
| S16A | 9.1 (1) | 40.5 (5) | 80.8 (33) | 97.0 (3) | 91.8 (24) | 101.0 (31) | 89.9 (13) | 93.9 (35) | 104.1 (6) |
| W20A | 9.9 (1) | 33.4 (6) | 74.7 (13) | 76.0 (2) | 76.3 (17) | 73.3 (37) | 57.8 (16) | 52.7 (28) | 99.2 (19) |
| W101A | 60.4 (15) | 75.9 (19) | 69.0 (13) | 60.1 (11) | 12.9 (9) | 4.6 (10) | 67.0 (10) | 91.5 (29) | 3.7 (4) |
| F108A | 81.5 (5) | 92.2 (17) | 85.2 (12) | 71.7 (18) | 1.7 (0) | 32.3 (22) | 88.7 (0) | 86.0 (21) | 101.7 (7) |
| I170A | 72.7 (10) | 73.6 (10) | 3.1 (4) | 6.1 (6) | 70.7 (18) | 75.6 (25) | 42.4 (6) | 49.3 (18) | 100.3 (9) |
| E184A | 44.6 (5) | 41.9 (2) | 0.3 (1) | 2.9 (5) | 62.7 (5) | 74.3 (22) | 29.2 (2) | 41.0 (3) | 84.4 (9) |
| R188A | 20.6 (17) | 94.8 (13) | 26.2 (2) | 66.0 (1) | 98.3 (4) | 90.9 (16) | 59.0 (15) | 94.7 (2) | 111.9 (12) |
| K284A | 151.4 (3) | 99.1 (23) | 17.5 (1) | 83.9 (9) | 75.8 (21) | 82.2 (12) | 72.5 (6) | 67.9 (2) | 84.3 (21) |
Summary of binding data for the anti-DENV mAbs and Fabs is shown. Ab reactivities for each alanine scan mutant are expressed as percent of wild-type with ranges (half of the maximum minus minimum values) in parentheses. At least 2 replicate values were obtained for each experiment.