Table 4.

mAB Epitope mapping

mAb	Binding to 80E types	Neutralizing Dengue types	Mapping region	Residue locations
DC4	Type 2	Type 2	Domain I	I170, E184, R188, K284
DB5	Types 1, 2, 3	Types 1, 2, 3	Domain I	S16,W20
DDIL	Types 1, 2, 3, 4	Types 1, 2, 3, 4	Fusion Loop	W101,F108

Summary of epitope mapping results from 3 dengue-specific mAb with disparate binding and neutralization properties. Binding to 80E proteins were measured in 80E specific ELISA assays, as shown in Figure 5 , and viral neutralization as measured in the infrared dengue neutralization assay , as listed in Table 2. Critical residues for loss of antibody binding were determined by shotgun mutagenesis.