Table 2.
List of F. tularensis proteins isolated on magnetic SiMAG-Hydrazide microspheres
| Gene locusa | Protein name | (Mwb/kDa)/pI | PSORTbc | LipoPd |
|---|---|---|---|---|
| FTH_0010 | Sulfur transferase | 15.89/9.22 | ? | cyt |
| FTH_0038 | Probable multidrug efflux pump | 37.24/9.17 | CM | TMH |
| FTH_0039 | Putative uncharacterized protein | 15.30/5.16 | ? | SPII |
| FTH_0066 | 30S ribosomal protein S20 | 10.15/10.96 | cyt | cyt |
| FTH_0067 | Elongation factor 4 | 65.57/5.42 | CM | cyt |
| FTH_0108 | Conserved protein PdpC | 156.00/9.17 | cyt | cyt |
| FTH_0117 | Conserved protein (conserved protein PdpB) | 127.47/9.63 | OM | cyt |
| FTH_0124 | FeoB family ferrous iron (Fe2+) uptake protein | 81.46/8.55 | CM | cyt |
| FTH_0139 | ABC superfamily ATP binding cassette transporter, ABC protein | 49.45/4.72 | CM | cyt |
| FTH_0159 | Universal stress protein | 30.4/5.78 | cyt | cyt |
| FTH_0169 | 50S ribosomal protein L34 | 5.18/12.96 | cyt | cyt |
| FTH_0184 | Cytochrome d ubiquinol oxidase subunit I | 64.28/7.63 | CM | TMH |
| FTH_0186 | Cytochrome o ubiquinol oxidase subunit II | 34.46/7.02 | CM | TMH |
| FTH_0187 | Cytochrome o ubiquinol oxidase subunit I | 76.20/8.58 | CM | TMH |
| FTH_0189 | Cytochrome o ubiquinol oxidase subunit IV | 12.19/6.37 | CM | TMH |
| FTH_0219 | Ribosomal protein S2 | 26.42/8.94 | cyt | cyt |
| FTH_0228 | Ribosomal protein S7 | 17.82/10.82 | cyt | cyt |
| FTH_0230 | Ribosomal protein S10 | 11.90/9.79 | cyt | cyt |
| FTH_0231 | Ribosomal protein L3 | 22.31/9.65 | cyt | cyt |
| FTH_0232 | 50S ribosomal protein L4 | 22.55/10.29 | cyt | cyt |
| FTH_0234 | Ribosomal protein L2 | 30.40/11.54 | cyt | cyt |
| FTH_0237 | Ribosomal protein S3 | 24.88/10.10 | cyt | cyt |
| FTH_0238 | Ribosomal protein L16 | 15.71/11.43 | cyt | cyt |
| FTH_0239 | Ribosomal protein L29 | 7.80/10.34 | cyt | cyt |
| FTH_0241 | Ribosomal protein L14 | 13.23/10.51 | cyt | cyt |
| FTH_0242 | 50S ribosomal protein L24 | 11.48/9.73 | cyt | cyt |
| FTH_0243 | Ribosomal protein L5 | 20.00/9.75 | cyt | cyt |
| FTH_0246 | Ribosomal protein L6 | 19.08/9.85 | cyt | SPI |
| FTH_0248 | Ribosomal protein S5 | 17.56/9.99 | cyt | cyt |
| FTH_0250 | Ribosomal protein L15 | 15.10/10.24 | cyt | cyt |
| FTH_0251 | Preprotein translocase subunit secY | 48.46/10.01 | CM | TMH |
| FTH_0253 | Ribosomal protein S13 | 13.38/11.54 | cyt | cyt |
| FTH_0254 | Ribosomal protein S11 | 13.76/10.85 | cyt | cyt |
| FTH_0255 | Ribosomal protein S4 | 23.26/9.85 | cyt | cyt |
| FTH_0257 | Ribosomal protein L17 | 16.78/10.72 | cyt | cyt |
| FTH_0268 | Glutamate dehydrogenase (NADP(+)) | 49.16/6.49 | ?, MLS | cyt |
| FTH_0295 | Acetyl-CoA carboxylase α subunit | 35.44/8.07 | cyt | cyt |
| FTH_0310 | Pyruvate dehydrogenase (acetyl-transferring) | 100.27/5.65 | cyt | cyt |
| FTH_0334 | Probable OmpA family protein | 23.29/4.95 | OM | SPII |
| FTH_0357 | LemA family protein | 21.97/5.63 | cyt | cyt |
| FTH_0384 | Type IV pili fiber protein (PilA) | 13.59/9.06 | ?, MLS, fimbrial | SPI |
| FTH_0384 | Type IV pili fiber protein (PilA) | 13.59/9.06 | ?, MLS, fimbrial | SPI |
| FTH_0403 | Putative uncharacterized protein | 32.50/8.46 | cyt | cyt |
| FTH_0447 | Phosphatidylserine decarboxylase | 32.13/9.72 | CM | cyt |
| FTH_0462 | Possible neuraminidase | 42.6/9.52 | CM | TMH |
| FTH_0463 | Soluble lytic murein transglycosylase | 76.93/9.09 | PP | SPI |
| FTH_0515 | Cell division topological specificity factor protein MinE | 10.18/8.03 | cyt | cyt |
| FTH_0516 | Septum site-determining protein MinD | 30.8/6.85 | cyt, MLS | cyt |
| FTH_0519 | Ribosomal protein L28 | 8.94/11.06 | cyt | cyt |
| FTH_0535 | DNA topoisomerase (ATP-hydrolyzing) | 97.13/5.41 | cyt | cyt |
| FTH_0539 | UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase | 35.8/5.80 | cyt | cyt |
| FTH_0540 | (3R)-Hydroxymyristoyl-[acyl-carrier-protein] dehydratase | 18.15/6.83 | cyt | cyt |
| FTH_0541 | Acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase | 28.13/8.37 | cyt | cyt |
| FTH_0542 | 1,4-α-Glucan branching enzyme | 43.09/9.34 | cyt | cyt |
| FTH_0554 | Signal peptidase I | 32.87/8.82 | CM | TMH |
| FTH_0585 | Acyl-CoA dehydrogenase | 84.19/8.45 | ? | cyt |
| FTH_0586 | Long-chain-fatty-acid–CoA ligase | 63.07/7.57 | CM | cyt |
| FTH_0589 | Putative uncharacterized protein | 33.26/9.53 | CM | TMH |
| FTH_0592 | dTDP-glucose 4,6-dehydratase | 65.71/8.17 | CM | TMH |
| FTH_0593 | Galactosyltransferase | 23.78/10.21 | CM | SPI |
| FTH_0594 | UDP-glucose 4-epimerase | 29.96/9.46 | ? | SPI |
| FTH_0595 | Galacturonosyltransferase | 41.60/9.39 | cyt | cyt |
| FTH_0600 | Asparagine synthase (glutamine-hydrolyzing) | 71.81/7.91 | cyt | cyt |
| FTH_0604 | Glycosyltransferase | 32.98/6.51 | cyt | cyt |
| FTH_0628 | Possible glycosyltransferase | 35.58/7.16 | CM | cyt |
| FTH_0659 | Conserved hypothetical protein | 4.51/8.90 | cyt | TMH |
| FTH_0689 | Probable multidrug resistance efflux pump | 37.83/9.36 | ? | cyt |
| FTH_0719 | Ribonuclease E | 95.95/7.12 | cyt | cyt |
| FTH_0799 | Bifunctional 1-pyrroline-5-carboxylate dehydrogenase/proline dehydrogenase | 150.39/7.97 | cyt | cyt |
| FTH_0800 | APC family amino acid–polyamine-organocation transporter | 55.00/9.27 | CM | TMH |
| FTH_0836 | Preprotein translocase subunit | 12.87/10.55 | CM | SPI |
| FTH_0837 | RND superfamily resistance-nodulation-cell division antiporter | 69.67/9.52 | CM | TMH/SPI |
| FTH_0886 | Probable membrane protease subunit HflK | 40.42/8.97 | cyt | cyt |
| FTH_0887 | Membrane protease subunit HflC | 34.59/9.97 | ? | cyt |
| FTH_0928 | 50S ribosomal protein L25 | 10.90/9.26 | cyt | cyt |
| FTH_1002 | Ribosomal protein L9 | 16.45/5.55 | cyt | cyt |
| FTH_1026 | Ribosomal protein S21 | 7.84/11.4 | cyt | cyt |
| FTH_1071 | Thioredoxin family protein | 39.69/4.89 | ? | SPII |
| FTH_1078 | Putative uncharacterized protein | 16.87/7.40 | ? | TMH |
| FTH_1107 | Inositol-phosphate phosphatase | 28.87/8.87 | cyt | cyt |
| FTH_1112 | 3-Oxoacyl-[acyl-carrier-protein] synthase | 44.3/5.62 | cyt | cyt |
| FTH_1117 | Fatty acid/phospholipid synthesis protein PlsX | 37.84/9.62 | cyt | cyt |
| FTH_1162 | Ribosomal protein S9 | 10.09/10.35 | cyt | cyt |
| FTH_1163 | Ribosomal protein L13 | 15.94/10.03 | cyt | cyt |
| FTH_1197 | Aminotransferase | 47.07/9.06 | cyt | cyt |
| FTH_1216 | Sec family Type II general secretory pathway signal recognition particle protein Ffh | 50.31/10.31 | CM | cyt |
| FTH_1267 | Oxidoreductase | 30.80/9.33 | cyt | cyt |
| FTH_1281 | Acetyl-CoA carboxylase β subunit | 33.65/8.96 | cyt | cyt |
| FTH_1354 | ATP-dependent RNA helicase | 64.01/8.91 | cyt | cyt |
| FTH_1366 | Ribosomal protein L20 | 13.34/10.81 | cyt | cyt |
| FTH_1377 | Putative uncharacterized protein | 44.26/9.77 | ? | TMH |
| FTH_1379 | Probable capsule biosynthesis protein | 44.90/7.41 | CM | TMH |
| FTH_1415 | Ribosomal protein L21 | 11.56/10.04 | cyt | cyt |
| FTH_1418 | Sec family Type II general secretory pathway preprotein translocase SecA subunit | 103.56/5.29 | cyt, MLS | cyt |
| FTH_1424 | M41 family endopeptidase FtsH/HflB | 70.74/5.42 | CM | TMH |
| FTH_1443 | Bifunctional FAD/FMN dehydrogenase/Fe–S oxidoreductase | 114.57/8.64 | cyt | cyt |
| FTH_1487 | Polyribonucleotide nucleotidyltransferase | 75.53/5.68 | cyt | cyt |
| FTH_1488 | Ribosomal protein S15 | 10.36/10.13 | cyt | cyt |
| FTH_1489 | Peptidoglycan glycosyltransferase | 62.73/8.77 | CM | TMH |
| FTH_1492 | Probable acyl-coenzyme A synthetase | 78.58/9.04 | CM | cyt |
| FTH_1503 | Conserved hypothetical protein | 69.85/10.14 | CM | TMH |
| FTH_1536 | Conserved hypothetical protein | 25.35/6.96 | ? | cyt |
| FTH_1537 | Acetyl-CoA carboxylase biotin carboxylase subunit | 50.05/6.85 | cyt | cyt |
| FTH_1551 | S49 family SohB endopeptidase | 38.07/9.25 | CM | TMH |
| FTH_1558 | Glycosyltransferase, group 2 family protein | 36.08/8.69 | CM | cyt |
| FTH_1599 | Conserved hypothetical protein | 49.32/4.33 | ?, MLS | SPI |
| FTH_1609 | ABC superfamily ATP binding cassette transporter, ATP-binding protein | 66.68/8.69 | CM | SPI |
| FTH_1612 | RND family efflux transporter, MFP subunit | 50.09/10.12 | CM | cyt |
| FTH_1613 | RND superfamily resistance-nodulation-cell division: proton (H+) antiporter | 112.50/5.40 | CM | TMH |
| FTH_1617 | Conserved hypothetical protein | 38.46/9.50 | CM | SPI |
| FTH_1642 | Cell division protein | 92.02/5.63 | CM | TMH |
| FTH_1674 | Ribosomal protein L19 | 13.31/10.53 | cyt | cyt |
| FTH_1677 | Ribosomal protein S16 | 9.07/10.61 | cyt | cyt |
| FTH_1682 | DNA-directed RNA polymerase subunit β′ | 157.39/5.97 | cyt | cyt |
| FTH_1687 | Ribosomal protein L1 | 24.63/9.50 | cyt | cyt |
| FTH_1691 | Elongation factor Tu | 43.39/4.87 | cyt | cyt |
| FTH_1696 | Glycerol-3-phosphate dehydrogenase | 57.78/8.36 | ?, MLS | cyt |
| FTH_1708 | Aconitate hydratase | 102.70/5.30 | cyt | cyt |
| FTH_1719 | Dihydrolipoyllysine-residue succinyltransferase | 52.75/4.89 | cyt | cyt |
| FTH_1720 | Oxoglutarate dehydrogenase (succinyl-transferring) | 105.67/6.01 | cyt | cyt |
| FTH_1721 | Succinate dehydrogenase | 27.2/8.42 | CM | cyt |
| FTH_1722 | Succinate dehydrogenase | 65.86/6.14 | CM | cyt |
| FTH_1726 | MFS family major facilitator transporter | 47.16/9.78 | CM | TMH |
| FTH_1732 | H(+)-transporting two-sector ATPase | 49.87/4.89 | cyt | cyt |
| FTH_1734 | H(+)-transporting two-sector ATPase | 55.54/4.68 | cyt | cyt |
| FTH_1736 | H(+)-transporting two-sector ATPase | 17.38/7.54 | CM | cyt |
| FTH_1738 | ATP synthase subunit a (ATP synthase F0 sector subunit a) | 30.00/6.22 | CM | cyt |
| FTH_1760 | NADH dehydrogenase (ubiquinone) | 87.36/5.09 | cyt, MLS | cyt |
| FTH_1761 | NADH dehydrogenase (ubiquinone) | 46.8/5.61 | cyt, MLS | cyt |
| FTH_1763 | NADH dehydrogenase (ubiquinone) | 47.59/7.06 | cyt, MLS | cyt |
| FTH_1824 | Conserved hypothetical protein | 20.32/6.64 | CM | TMH |
| FTH_1830 | Cell division protein FtsZ | 39.8/4.76 | cyt, MLS | cyt |
| FTH_1831 | Cell division protein FtsA | 44.80/5.20 | cyt | cyt |
| FTH_1875 | N-(5′-Phosphoribosyl)anthranilate isomerase | 51.32/8.86 | cyt | cyt |
a
The accession number in the genome sequence of F. tularensis subsp. holarctica OSU18.
b
Theoretical molecular weight/theoretical pI.
c
Prediction of the protein localization using the PSORTb program, cyt—cytoplasmic, CM cytoplasmic membrane, OM—outer membrane, PP—periplasm, EC—extracellular space, ?—unknown localization, MLS—multiple localization sites.
d
Prediction of lipoproteins (SPII cleavage site II) and SPI (cleavage site I) using the LipoP algorithm, TMH—transmembrane domain.