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EV–MNP complexes isolated from the blood of ACS patients and healthy volunteers using CD31 marker. EVs from ACS patients (1) and healthy volunteers (2) isolated using MNPs coupled to antibodies against CD31 were stained with fluorescently labeled antibodies against CD41a and CD63. Stained EV–MNP complexes were isolated on magnetic columns and phenotyped by flow cytometer. The p value was calculated using the Mann–Whitney test.
