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. Author manuscript; available in PMC: 2016 Jul 29.
Published in final edited form as: Methods Mol Biol. 2015;1288:395–412. doi: 10.1007/978-1-4939-2474-5_23

Figure 3. Analysis of transcription through 603 nucleosome containing intact and fluorophore-labeled DNA.

Figure 3

Transcription by the RNAP was conducted in the presence of NTPs for 30 seconds at 150 mM KCl. The locations of the 603 nucleosome (oval), labeled DNA (*) and the run-off transcript are shown. No additional pausing was detected on fluorophore-labeled DNA, suggesting that fluorophores do not interfere with progression of the enzyme.