Skip to main content
. Author manuscript; available in PMC: 2016 Jul 29.
Published in final edited form as: Nat Microbiol. 2016 Feb 15;1:16001. doi: 10.1038/nmicrobiol.2016.1

Figure 2. Detection of free T. gondii tachyzoites in the vascular compartment.

Figure 2

C57BL/6 mice were infected with 1 × 105 T. gondii tachyzoites expressing tdTomato, and the blood was analysed by flow cytometry. a, Representative flow cytometry plots of tdTomato versus forward scatter (FSC) as a measure of cell size show the ability to distinguish free parasites from infected cells. Parasites isolated from tissue culture are shown in the first panel and have a low FSC and are detected in a tight left gate. Cell associated parasites have a high FSC and are present in a broader right gate. They can be detected in infected mice starting at day 4 post infection, but are absent by day 10. b, Kinetics and quantification of free and cell-associated parasites in the blood. Lymphocyte-deficient (RAG) mice and secretory IgM-KO (sIgM) mice are unable to control the parasite burden. Data are from three independent experiments and each dot represents a single mouse (n = 20 mice per experiment).