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. 2016 Jul 29;9:422. doi: 10.1186/s13071-016-1704-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Characterization of Hc-daf-22 gene from H. contortus. a Alignment of the deduced amino-acid sequences of Hc-DAF-22 and other species. Protein sequences were aligned using CLUSTAL W and BoxShade. Shading in black indicates residues that are consistent. Red boxes represent thiolase acyl-enzyme intermediate signatures, the first box represents the thiolases aryl-enzyme intermediate signature and the second box represents the thiolase-2 thiolase signature. The accession numbers representing these sequences are: NP 496639.1 (C. elegans), EYC23922.1 (A. ceylanicum: Ancylostoma ceylanicum), XP 001902124.1 (B. malayi: Brugia malayi), CEF60652.1 (S. ratti: Strongyloides ratti), XP_013305621.1 (N. americanus: Necator americanus), KHJ80319.1 (O. dentatum: Oesophagostomum dentatum), KHN79232.1 (T. canis: Toxocara canis), XP_002065586.1 (D. willistoni: Drosophila willistoni), NP_957159.1 (D. rerio: Danio rerio), NP_001292129.1 (G. gallus: Gallus gallus), NP_035457.1 (M. musculus: Mus musculus), NP_001075504.1 (O. cuniculus: Oryctolagus cuniculus), NP_001007099.1 (H. sapiens: Homo sapiens). b Phylogenetic tree based on deduced amino acid sequences of Hc-DAF-22 and its homologues from other species. NP 496639.1, AAB06496.1 (C. elegans), EYC23922.1 (A. ceylanicum: Ancylostoma ceylanicum), XP 001902124.1 (B. malayi: Brugia malayi), CEF60652.1 (S. ratti: Strongyloides ratti), XP_013305621.1 (N. americanus: Necator americanus), XP_002065586.1 (D. willistoni: Drosophila willistoni), NP_957159.1 (D. rerio: Danio rerio), NP_001292129.1 (G. gallus: Gallus gallus), NP_035457.1 (M. musculus: Mus musculus), NP_001007099.1 (H. sapiens: Homo sapiens)