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. 2016 Jul 29;9:422. doi: 10.1186/s13071-016-1704-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Hc-DAF-22 is located in intestinal cells in C. elegans N2 worms using Ce-daf-22 promoter. The plasmid vector CeP-pPD95.77-Hc-daf-22 which contained the 2,061 bp region upstream of the Ce-daf-22 initiation codon and 1,105 bp region of the Hc-daf-22 coding sequence was constructed and injected into wild type N2 worms. The expression pattern of Hc-DAF-22 in the N2 strain is shown, GFP could be seen throughout the whole intestine in both larvae and adults with high intensity. a11-a33 L4 larvae; b11-b33 adult worms. Scale-bars: 200 μm