Table 1.
Primers used in all experiments
| Primer ID and direction | Primer sequence 5′–3′ |
|---|---|
| Hc-daf-22 1F | TACAGACAGTGCCCGGCCATCA |
| Hc-daf-22 1R | GGCAGTAATAGGGGCAGGGAACA |
| Hc-daf-22 2F | GCTTCTGGCTCTTCTGGGCTCTACT |
| Hc-daf-22 2R | GAAAGCGGTAACTCTGACGTTGTGCTTGC |
| Hc-daf-22 F1 | ATGGGTAAATCAAAAGTATATG |
| Hc-daf-22 R1 | ACTGAGTGCAGATGGTT |
| Hc-daf-22 F2 | ACGCCGGCAAAGAACATAT |
| Hc-daf-22 R2 | CCCTTACCGACGTCACACACAAT |
| Hc-daf-22 F3 | ACTTATGAGGCTCTGGGATTG |
| Hc-daf-22 R3 | GCATCGATAGTCCACTTCTTTAC |
| Hc-daf-22 F4 | AGGAAAATGGGTGATCAATCCGTCT |
| Hc-daf-22 R4 | TCAGATTTTCGCTTTGAGCATTTTC |
| Hc-daf-22-SP1 | GATAGGTATGCCAGTGAACCCGAGT |
| Hc-daf-22-SP2 | GCAATCGTCCAACGCCATATTCACT |
| Hc-daf-22-SP3 | AATACACTCACCGGCTTCCTTCACC |
| Hc-daf-22 RT F | GTATATGTGATTGGTGTCGG |
| Hc-daf-22 RT R | AATATCCGACGGTAGCTT |
| tub-F | TGTTCCATCACCCAAGGTATCC |
| tub-R | TGACAGACACAAGGTGGTTGAGAT |
| Hc-daf-22PF | AACTGCAGGATTGGTCGAGAGAGATGAAAC |
| Hc-daf-22PR | CGGGATCCGATGGTCCGGGCTGCAATAAATT |
| Hc-daf-22dF | TCCCCCGGGATGGGTAAATCAAAAGTATATG |
| Hc-daf-22dR | GGGGTACCCAGATTTTCGCTTTGAGCAT |
| Ce-PF | GCGCTCTAGATTAATTCAATGCACAACTC |
| Ce-PR | TCCCCCGGGTTTCTGGAACAATATT |
| RNAi F | CCCAAGCTTGTGAACAATGCCTGCCTGCGCTT |
| RNAi R | CTAGCTAGCCCTGTCGCACCGATGGG |