Fig. 4.

Determination of the regulatory pathway of PADI2 using PCR arrays. The tumor cell line MCF-7 was treated with anti-PADI2 siRNA. Cells treated with Allstar siRNA were used as a negative control. a PADI2 expression level was detected using real time-PCR in MCF-7 cells following treatment with anti-PADI2 siRNA. The PADI2 transcription level in the treated cells was normalized with the mRNA level in negative control cells. b Cancer pathway finder (c) Signal Transduction PCR arrays were used to detect altered expression of tumor-related genes in the treated cells. Fold changes were calculated and expressed as log-normalized ratios of the expression level in siRNA-treated cells/the expression level in negative control. Genes with at least a fourfold change in expression were considered biologically significant