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. 2016 Jan 29;8(2):253–263. doi: 10.1080/19420862.2015.1128605

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Healthy donor IL-2 ELISpot responses to IgG1 G1m3-trastuzumab and G1m1,17-trastuzumab. Replicate cultures were incubated in the presence of test samples for a total of 8 days (in parallel to the proliferation assays) prior to detection of secreted IL-2. The threshold for positive IL-2 ELISpot responses (SI ≥ 1.90, p < 0.05) is indicated by the dotted line. Borderline responses (SI = 1.90-1.95, p < 0.05) are indicated (*). (a) homozygous G1m3 donors and (b) homozygous G1m1,17 donors. No ELISpot data was obtained from donors 32 and 49.